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Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
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Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
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Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
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Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
-
Product PartDescription:
The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
OUT OF STOCKThe effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel as a base material in the STR series. STR series columns are durable due to the use of mechanically strong silica gel. STR series columns are highly resistant to acidic and basic mobile phases. STR series columns are easy to operate and display high levels of performance due to low analytical operating pressures. Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals.
C18 Reversed Phase Columns in Singapore
A C18 column is a fundamental component of High-Performance Liquid Chromatography (HPLC), a technique for separating and analysing liquid compounds. As the most widely used type of reversed-phase column, it features a stationary phase made from non-polar C18 material, essential for separating compounds based on their hydrophobic properties.
These columns, with applications in pharmaceuticals, environmental science, and chemical analysis, are available in various sizes and particle types for precise customisation. To meet the stringent demands of modern analytical techniques, Shimadzu offers a comprehensive range of advanced C18 columns engineered for exceptional performance and reliability.
Understanding the Types of C18 Reversed Phase HPLC Columns
While all C18 columns are used for reversed-phase chromatography, they are not all the same. They can differ in silica particle type, particle pore size, particle surface area, carbon loading, end-capping, and bonding technology. These factors result in unique separation profiles and applications for the same mixture of compounds. Here are some of the major types of C18 columns:
Arata C18
Engineered for unprecedented resolution and superior peak shape, this column is ideal for both basic and acidic compounds. It also offers rapid equilibration, which is beneficial for LC/MS analysis.
GIST C18
Known for its superior inertness and wide pH range, the GIST C18 column enhances analysis precision and column stability. It is well-suited for samples that are difficult to analyse, providing symmetrical peaks.
GISS C18
This ultra-high inertness column is designed for rapid separations with symmetrical peaks, making it a great choice for LC/MS/MS analysis. It is optimised with a large surface area and pore size for superior performance.
GIS C18
This column features strong hydrophobic interaction and low adsorption of ionic compounds. Its highly uniform particles ensure stable mobile phase delivery and low pressure, making it ideal for preparative separations.
Velox C18
Designed as a general-purpose reversed-phase column, the Velox C18 offers the highest hydrophobic retention among the Velox phases. It is suitable for a wide range of applications in various industries.
Solar C18
Packed with high-purity, fully end-capped silica gel, the Solar C18 column provides good hydrophobic retention and excellent peak shapes. It has a wide pH range, making it suitable for versatile method development.
Besides these, Shimadzu offers an even wider array of specialised C18 columns to meet every need, including the ShimNex WP C18, ShimNex UP C18, and NovaCore C18.
Applications of C18 Columns
Reversed-phase C18 HPLC columns have wide-ranging applications in various industries, including pharmaceuticals, environmental monitoring, and natural product analysis.
In the pharmaceutical industry, C18 columns are indispensable for analysing drug substances, formulations, and for use in pharmacokinetic studies. Their selectivity allows for the separation and quantification of diverse pharmaceutical compounds, ensuring compliance with regulatory standards. With the ability to resolve closely related compounds, including impurities and degradation products, C18 columns play a vital role in drug development, quality control, and stability testing.
Environmental monitoring involves identifying and quantifying organic contaminants in water, soil, and air samples. Reversed-phase C18 columns facilitate the analysis of environmental pollutants such as pesticides, herbicides, and industrial chemicals. Leveraging the selectivity of C18 columns, these contaminants can be accurately detected and quantified even at trace levels. This analytical capability aids in environmental risk assessment and the formulation of appropriate mitigation strategies.
For natural product analysis, C18 columns are instrumental in studying plant extracts, herbal medicines, and essential oils. Natural products often contain a wide array of compounds with diverse polarities, posing challenges in their separation and characterisation. C18 columns excel in separating complex mixtures based on hydrophobic interactions, enabling the identification and quantification of individual components. This analytical capability contributes to quality control, standardisation, and the exploration of potential therapeutic applications.
C18 Columns for Precision & Accuracy
When it comes to achieving precision and accuracy in analytical separations, Shimadzu's C18 reversed-phase columns are the epitome of excellence. These columns are specifically designed to deliver exceptional performance, ensuring reliable and reproducible results in a wide range of applications.
1. Excellent Reproducibility
Our C18 columns exhibit outstanding reproducibility, allowing for consistent and reliable results across multiple runs. This exceptional reproducibility is crucial for method validation and quality control, providing the confidence needed in analytical outcomes.
2. Column Stability
Our C18 columns are also built with utmost durability and stability, maintaining peak performance even under demanding conditions. The columns are designed to withstand high pressures and maintain consistent separation efficiency, ensuring longevity and reliability in chromatographic analyses.
3. Wide Range of Particle and Pore Sizes
We offer a diverse selection of C18 columns with varying particle sizes and pore structures to meet the specific needs of different applications. This flexibility allows for optimised separations, improved resolution, and enhanced sensitivity, accommodating a wide range of sample complexities.
4. Excellent Peak Shape and Resolution
Our C18 columns are engineered to deliver exceptional peak shapes, providing sharp and well-defined chromatographic peaks. This attribute is crucial for accurate quantification and reliable identification of target compounds, ensuring precise and reliable analytical results.
5. Compatibility with a Variety of Analytical Methods
Our C18 columns exhibit exceptional versatility and compatibility, enabling seamless integration with a wide range of analytical methods. Whether you are performing HPLC, UHPLC, LC-MS, or other techniques, our C18 columns deliver consistent and reliable separations, supporting diverse analytical workflows.
Get C18 Columns in Singapore from Shimadzu
Finding a C18 column that is right for your application is important to ensure good sample separation and peak resolution. At Shimadzu, we provide a wide selection of reversed-phase C18 HPLC columns to cater to your application needs. Shimadzu HPLC columns are manufactured and tested individually to ensure superior quality and inertness, giving you high precision in analysis and increased column durability. In addition, a highly reproducible symmetrical peak shape can be achieved even when analysing strong ionic compounds and difficult to absorb samples. Shimadzu also offers suitable C18 columns for the analysis of highly polar compounds under 100% aqueous mobile phase, with excellent retentivity.
For other C18 columns selection, feel free to contact us. Alternatively, browse our range of HPLC columns and lab consumables at ShopShimadzu, and register for an account to purchase our products online.
Frequently Asked Questions About C18 Columns in Singapore
1. Can I use 100% aqueous mobile phase on the C18 column?
Not all C18 columns are stable to 100% aqueous mobile phase conditions. Using a 100% aqueous mobile phase on a C18 column that cannot withstand such conditions will result in a phenomenon called phase collapse that damages the column. Some of Shimadzu’s C18 columns that are compatible with 100% aqueous conditions are Shim-pack Scepter C18, Shim-pack GIST C18-AQ, Shim-pack GIS RP-Shield and Shim-pack GISS C18.
2. What are the differences between a C8 and a C18 column?
The main difference between C8 and C18 columns is their hydrophobicity and retention strength. A C18 column, with its longer 18-carbon chain, is more hydrophobic, making it the standard choice for retaining and separating a wide range of non-polar compounds. In contrast, a C8 column has a shorter 8-carbon chain and is less hydrophobic. This results in weaker retention and shorter analysis times, making it a better option for separating highly hydrophobic samples that might be too strongly retained on a C18 column.
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