Injection Port Septum

Even when a low bleed septum is used, bleeding is not totally eliminated. The type of bleeding differs according to the septum type and bleeding appears as different peaks on a chromatogram. In high-sensitivity analysis, you need to select and use a septum that does not cause bleeding at a position where interference occurs with the target compound.

When using an autosampler, it is recommended to exchange the septum after about 100 injections. If the injection is done manually or the outer diameter of the needle is thick, the septa should be replaced more often.

Part Number/Description	Pack Size	Color	Description
227-35511-01 Xtra Life Inlet Septa	25	Red	Extra long-life septum, lasts up to 10x longer than a standard septum. Maximum temperature (INJ setting temperature): 400°C
227-35004-01 Premium Green Septa	50	Light green	Useful for trace analysis and other applications where high sensitivity is critical Low bleed, highly robust (max. usable temp. 350°C) High durability, proper sealing and good resistance to most chemical solvents.
221-75180 Enduro Blue Septum	50	Light blue	Low-bleed, suitable for high-sensitivity analysis at high temperatures Maximum temperature (INJ setting temperature): 350°C
201-35584 Standard type	20	White	General-purpose septum Maximum temperature (INJ setting temperature): 250 °C
221-48972-91 long life type	20	Blue	Provides signiﬁcant durability improvements compared to a conventional low-bleed septum, offering both low bleed and long life. The problem of sticking to the vaporizing chamber during continuous use at high temperatures experienced with a conventional septum has also been eliminated. Suitable for high-sensitivity analysis Maximum temperature (INJ setting temperature): 450°C
221-48398-91 high temp type	20	Brown	Using this septum alleviates the problem of reduced durability when the vaporizing chamber is used continuously at 450°C. Compared to the LL septum, the increase in bleed when used at high temperatures is kept at a lower level. The problem of sticking to the vaporizing chamber during continuous use at high temperatures experienced with a conventional septum has also been eliminated. Suitable for high-sensitivity analysis at high temperatures Maximum temperature (INJ setting temperature): 450°C
221-76650-01 Low-bleed Septum	25	Green	This septum is least inﬂuenced by a plasticizer. Better prevents septum coring. Low-bleed, suitable for high-sensitivity analysis Maximum temperature (INJ setting temperature): 340°C
221-76863-96 Perforated septum for HS-10	1	White	For HS-10 only To ensure cleanliness and inertness Maximum temperature (INJ setting temperature): 250 °C